3-keto-bisnor-4-cholen-11 alpha, 22-diol and esters



r} genic, testoid, folliculoid, and progesterone ac- OFFICE S PT 3-KETO-BISNOR-4OHOLEN-110:,22-DI0 AND ESTERS Herbert 0. Murray, Hickory Corners, and Durey H. Peterson, Kalamazoo, Mich., assignors to The Upjohn Company, Kalamazoo, Mich., a corporation of Michigan No Drawing. Application July 1, 1952, Serial No. 296,726

6 Claims. (C1. 260-397.45)

UNITED STAT 8 The present invention relates to 3-keto-bisnorof para-toluenesulfonic acid, and the thus-pro- 4-cholen-lla,22-diol and esters thereof. duced enamine is ozonized with ozone whereupon The novel compounds of the present invention the resulting ozonide is split with zinc in acetic are represented by the formula: acid to produce ll-dehydroprogesterone.

- The 3-ketobisnor-4-cholen-11a,22-diol may be CH3 utilized for the preparation of lla-hydroxyprogesterone [Peterson et al., J. A. 0.6., '74, 1871 (thereon (1952)] by reaction with chromium trioxide in acetic acid to yield 3,11-diketo-bisnori-cholenic RO- 1O acid and reacting the thus produced compound with ethyl mercaptan to produce the thiol ester of the 3,11-diketo-bisnor-4-cholenic acid. The thiol ester is treated with deactivated Raney nickel to form an aldehyde which is reacted with a seco 15 ondary amine, e. g., with piperidine in benzene in the presence of para-toluenesulfonic acid, and the thus-produced enamine is ozonized with wlierem R 15 hydrogen or carboxyhc acyl radl' ozone whereupon the resulting ozonide is split g s keto bisnor 4 Cho1en 11a 22 dio1 of the W1th zinc in acetic acid to produce lloc-hYdIOXY- 2O progesterone. irritants;retractin it e of w to of a iiitzart sires wtritttr rrsizt ture of fungus of the order Mucorales as described f 1 k t k t f O in Serial filed July 1952* now fe c ted aci i c z i d ac id ciilo ridz it a cid a ti- 25 or Augufit 19 1950 now abndoped seril NO in a solvent such as, for example, pyridine or the 5 2 d 23 5 su d J 1 like, or in an inert solvent, including solvents like 1952 Paient g g & 1S 8 u y benzene, toluene, ether, and the like, and heated It an object of the present invention to pre- 39 at 31 temperature bet-Ween about Zero degr-ees th I g keto bisnor cholen l1 22 diOl centigrade and the boiling pomt of the reaction pare enov mixture, usually about room temperature, for a and esters thereof. Other objects will be apparperiod between about a half-hour and about 96 g to skmed m the art to Whlch the mven' hours. The time of reaction may be varied acd f th t 35 cording to the temperature at which the reaction e compoun S 0 e plesen mven Ion is carried out, the particular acylating agent l m such as apaesthetic utilized, and the ratio of reactants used. The reand mhlbltory propertles m hypertenslve' estro' action mixture is suitably cooled with ice or cold Water, the product collected in an appropriate f' Furthermqe f 9 h solvent which is thereafter washed with succes- Invention are readuy punfieq enva'tlves of sive portions of a mildly basic solution and water z? keto'blsnor''cholen'llazz'dlol and are useful to obtain a solution of the roduct which is essenchemical iPtermediaies- The esters of the pres tially neutral. In some instances, the product Invention may be converted to may crystallize from the reaction mixture, in

progesterone [HegPer et 4 which case it may be advantageous to separate 715 (943)], havmg progestlonal actlvlty by the product by filtration or other means, wash pyrolysis of the ll-acylate to produce 22-acyloxywith Water, and thereafter purify by conventional b1snor-4,1l-cholad1ene-3-one followed by hydrolmeans such as for example by crystallization ysis of the 22-ester and oxidation of the resulting from Suitable Solvent or Chmmato m hie hydroxyl to yield 3-keto-bisnor-4,1l-choladienic 50 mm: d m d nee Th i acid. The resulting acid may be reacted with a ee 9 es ethyl mercaptan to produce the thiol ester of the scnbed acylatlon process prqduces both the mono-ester and the 11a,22-d1-ester, although in acid and the resulting thiol ester may be treated with deactivated Raney nickel to form an aldedlfierent DIODOTUOHS, p g p t e prohyde which is reacted with a secondary amine, portions of acyl e ge to 3- e -b S 1- e. g., with piperidine in benzene in the presence 5 cholen-11u,22-diol. W1th approximately one molar equivalent of acylating agent to said steroid there is produced predominantly the mono-acylated product, whereas with about two molar equivalents or more of acylating agent to said steroid, the predominate product is the diacylated product. If a mixed ester involving two different acyl groups is desired the 3-keto-bisnor- 4-ChOIGI'i-lla,22-dl01 may be partially esterified with one acylating agent and the resulting monoester may then be completely esterified with:an acylating agent which introduces a different acyl roup. Thus 11a-dimethylacetoxy-ZZ-,8-cyclo pentylpropionyloxy 3 keto bisnor 4 cholen or other mixed esters of the herein mentioned acid groups may be prepared.

Example 1.3-keto-bisnor-4-choZen-115:,22-diol' A medium was prepared of twenty grams of Edamine enzymatic digest of lactalbumin, three grams of' corn steep liquor. and fifty grams .of technical. dextrose diluted to one' liter with tap water and adjusted to a pH of 4.3. to 4.5. Twelve liters of this sterilized medium was inoculated with Rhizopus nigrz'cans. ATCC 6227b, and incubated for 24 hours at a temperature of 28 degrees centigrade using a rate of aeration and-stirring such that the oxygen uptake was 6.3 to 7 millimoles per hour per liter of .Na2SO3 according to the method of Cooper, Fernstrom-andMiller, Ind.

V 1 ethylene dichloride Eng. Chem, 36, 504 (1944). To this mediumcontaining a 24 hour growth ofRhizopus'nigricana minus strain was added three grams of- 3-ketobisnor-4-cho1en-22-al in 200 mililiters of ethanol to provide a suspension of the steroid in the culture. After an additional 48 hour periodof incubation under the same conditionsof temperature and aeration, the beer and-.mycelium were extracted. The mycelium was filtered, washed twice, each time with a volume of acetone. ap proximately equal to the volumeof the mycelium and extracted twice, each time with a volumeof methylene chloride approximately equal to the volume of the mycelium. The acetone and methylene chloride extracts including solvent were added to the beer. filtrate. The. mixed extracts and beer filtrate were extracted successively three times with three-liter portions of methylene chloride. The combined. methylene chlorideextracts were washed twice with 750 milliliters of.

two percent aqueous solution of sodium bicarbonate and twice with 750- milliliters of water. After drying the methylene. chloride extracts with about three to five gramsof anhydrous sodium sulfate per liter of solventand filtering, the.

solvent was removed by distillation to=produce 4.03 grams of semi-crystallineresidue. The extract was dissolved in 400 milliliters of ethylene dichloride and chromatographed over 320 grams of Florisil synthetic magnesium silicate using- 500-milliliter portions of developing solvents in accordance with Table I.

Fractions 11 to 16,. inclusive, were combined and recrystallized from ten millilitersiof=acetone by dropwise'addition of ether until'recrystallization was initiated. The crystals wereseparated and. again recrystallized from ten milliliters. of acetone to give 550.5 milligrams of 3.-keto-bisnor 4-cholen-l1a,22dio1, melting at 130 to 133 degrees centigrade. The infrared spectrum indicated the presence of two hydroxy groups andthe disappearance of the aldehyde group. The'optical rotation [(11 was plus 78 degrees (110175 in chloroform), and the ultraviolet extinction 76243 was 38.39.

TABLE I Eluatc Solids,

Solvent Milligrams Fraction do 3 ethylene dichloride-acetone 25:1

Hon FIDWOJQS pes-" 8 etbyenedichloride-acetone 12:1 o

ethyeno dichloride-a etone 10'1 Example 2 .1 1 a,22-diaceto:cy-3-ketobisnor-4-cholen To a 48 milligram sample of 3-keto-bisnor-4- ChOlEI'l-lloc,22dl0l dissolved in two milliliters of pyridine was added three milliliters of cold acetic anhydride and the reaction mixture maintained at room temperature for eighteen hours. The reaction mixture wasthereupon diluted with fifty milliliters of ice water, extracted three times with thirty milliliters of ether and the ether extracts washed three times with ten milliliters of five percent hydrochloric acid, three times with ten milliliters of five percent sodium hydroxide and five times with water. grams of sodium sulfate and evaporating at room temperature, 53 milligrams of crystals, having a melting point of to 126 degrees centigrade was obtained. Two recrystallizations from two milliliters of ether to which hexane was added, gave 38.5 milligrams'of 1-1a,22-diacetoxy-3-ketobisnori-cholen having a melting point of 127 to 128-degrees centigrade. The infrared spectrum showed that both hydroxy groups were acetylated. The optical rotation [M was plus 52 degrees (0.837 in chloroform) and the ultraviolet extinction 70242 was 33.95.

AnaZysis;-Calculated for 6261 13805: C, 72.52; H, 8.90. Found: C, 72.64; H, 8.73.

Example 3.-11a,22-dip'ropionyl0xy 3 keto-bisnor-4-cholen In the same. manneras given. in Example 2, 1104,22 dipropionyloxy-3-keto-bisnor-4-cholen is preparedusing an equivalent proportion of propionicanhydride in place of acetic anhydride.

Example 4-.-1'1,2z di(;8 cyclope'ntylpropionylomy) -3-keto-bisnor-4-cholen Example. 5.--11a,22 dibenzoxy 3 keto-bisnor- 4-cholen In the' same manner asgiven in- Example 2, 1112,22-dibenzoxy-B-keto-bisnor-4 cholen is prepared using equivalent proportions of benzoyl' chloride in place of acetic anhydride.

Upon drying over five Example 6.- 1111,22-dioctanoylomy-3-keto-bisnor- 4-cholen In the same manner as given in Example 2, 11m,22-dioctanoyloxy 3 keto-bisnor-l-cholen is prepared using equivalent proportions of octanoic anhydride in place of acetic anhydride.

In a similar manner other monoor (ii-esters of 3-keto-bisnor-4-cholen-11u,22-dio1 are prepared according to acylation procedures illustrated by the examples above or by reaction with ketene, ketenes of selected acids, selected acids, acid anhydrides, or acid chlorides, in an organic solvent such as pyridine or the like. Representative esters thus-prepared include one to eight carbon atom carboxylic acid acyloxy esters of saturated or unsaturated aliphatic, carbocyclic, cycloaliphatic, aryl, arylalkyl, alkaryl, mono-, di-, or poly-carboxylic acids which form ester groups such as, for example, formyloxy, acetoxy, propionyloxy, dimethylacetoxy, trimethylacetoxy, butyryloxy, valeryloxy, hexanoyloxy, cyclopentylformyloxy, acrylyloxy, cyclohexylformyloxy, cyclopentylpropionyloxy, the half and di-esters of malonic, maleic, succinic, glutaric, and adipic acids, and the like. The acids may also contain non-interfering substituents, such as mono or poly halo, chloro, bromo, hydroxy, methoxy, and the like.

It is to be understood that the invention is not 6 to be limited to the exact details of operation or exact compounds shown and described as obvious modifications and equivalents will be apparent to one skilled in the art and the invention is therefore to be limited only by the scope of the appended claims.

We claim:

1. A compound selected from the group consisting of 3-keto-bisnor-4-cholen-11a,22-diol and the 3-keto-bisnor-4-cholen-llu,22-dio1 esters of hydrocarbon carboxylic acids containing less than nine carbon atoms.

2. 1lm,22-diacetoxy-3-keto-bisnor-4-cholen.

3. 110;,22 dipropionyloxy 3 keto-bisnor-icholen.

4. 110;,22 di(;8 cyclopentylpropionyloxy) 3- keto-bisnor-4-cholen.

5. 11oz, 22-dibenzoxy-3-keto-bisnor-4-cholen.

6. 3-keto-bisnor-4-cholen-11,22-di0l.

References Cited in the file of this patent UNITED STATES PATENTS Name Date Cartland Apr. 6, 1943 OTHER REFERENCES Number 

1. A COMPOUND SELECTED FROM THE GROUP CONSITING OF 3-KETO-BISNOR-4-CHOLEN-11A,22-DIOL AND THE 3-KETRO-BISNOR-4-CHOLEN-11A,22-DIOL ESTERS OF HYROCARBON CARBOXYLIC ACIDS CONTAINING LESS THAN NINE CARBON ATOMS. 